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fitc labeled phalloidin  (Beijing Solarbio Science)


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    Structured Review

    Beijing Solarbio Science fitc labeled phalloidin
    Fitc Labeled Phalloidin, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 585 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phalloidin+fitc/FITC+Phalloidin/pmc13091063-315-21-37
    Average 96 stars, based on 585 article reviews
    fitc labeled phalloidin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: An Intelligent Magneto‐Mechanical Platform for Cellular Sensing in 3D Microenvironments
    Article Snippet: .. In order to label the filamentous actin (F‐actin), we incubated the cells with 1 μg/mL Phalloidin‐FITC (CA1620, Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4′,6‐diamidino‐2‐phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field‐emission scanning electron microscopy (FE‐SEM, Carl Zeiss, Germany).

    Article Title: Targeting JAK2 with a multifunctional nanoinhibitor for long-lasting anti-inflammatory effects and bone repair.
    Article Snippet: Following this period, the cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 for 10 min. Nonspecific binding sites were blocked with 5% goat serum for 1 h. Subsequently, cells were incubated overnight at 4 °C with anti-OCN (23418-1-AP, 1:400, Proteintech). .. The next day, cells were incubated with CoraLite594-conjugated secondary antibody (SA00013-4, 1:200, Proteintech) and Phalloidin-FITC (CA1620, 1:200, Solarbio) in the dark for 1 h. Images were captured using CLSM. ..

    Article Title: Finerenone ameliorates high-fat-induced myocardial lipotoxicity by suppressing ferroptosis through augmenting the system Xc - /GSH synthesis pathway.
    Article Snippet: Obesity cardiomyopathy (OCM) is a distinct clinical entity in cardiovascular disease, yet targeted therapeutic options are scarce.. Finerenone, a novel nonsteroidal mineralocorticoid receptor antagonist, has shown cardioprotective effects, but its role in mitigating OCM lipotoxicity is unclear.. To elucidate the underlying functions and mechanisms, rats were fed on an HFD for 16 weeks to establish the OCM model. At the 12th week of HFD, the OCM rats were administered 10 mg/kg finerenone for 4 weeks.

    Article Title: Targeting JAK2 with a multifunctional nanoinhibitor for long-lasting anti-inflammatory effects and bone repair
    Article Snippet: Following this period, the cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 for 10 min. Nonspecific binding sites were blocked with 5% goat serum for 1 h. Subsequently, cells were incubated overnight at 4 °C with anti-OCN (23418-1-AP, 1:400, Proteintech). .. The next day, cells were incubated with CoraLite594-conjugated secondary antibody (SA00013-4, 1:200, Proteintech) and Phalloidin-FITC (CA1620, 1:200, Solarbio) in the dark for 1 h. Images were captured using CLSM. ..

    Article Title: An Intelligent Magneto-Mechanical Platform for Cellular Sensing in 3D Microenvironments.
    Article Snippet: .. In order to label the filamentous actin (F-actin), we incubated the cells with 1 μg/mL Phalloidin-FITC (CA1620, 12 of 15 Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4 ′ ,6-diamidino-2-phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field-emission scanning electron microscopy (FE-SEM, Carl Zeiss, Germany).

    Labeling:

    Article Title: An Intelligent Magneto‐Mechanical Platform for Cellular Sensing in 3D Microenvironments
    Article Snippet: .. In order to label the filamentous actin (F‐actin), we incubated the cells with 1 μg/mL Phalloidin‐FITC (CA1620, Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4′,6‐diamidino‐2‐phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field‐emission scanning electron microscopy (FE‐SEM, Carl Zeiss, Germany).

    Article Title: An Intelligent Magneto-Mechanical Platform for Cellular Sensing in 3D Microenvironments.
    Article Snippet: .. In order to label the filamentous actin (F-actin), we incubated the cells with 1 μg/mL Phalloidin-FITC (CA1620, 12 of 15 Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4 ′ ,6-diamidino-2-phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field-emission scanning electron microscopy (FE-SEM, Carl Zeiss, Germany).

    Microscopy:

    Article Title: An Intelligent Magneto‐Mechanical Platform for Cellular Sensing in 3D Microenvironments
    Article Snippet: .. In order to label the filamentous actin (F‐actin), we incubated the cells with 1 μg/mL Phalloidin‐FITC (CA1620, Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4′,6‐diamidino‐2‐phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field‐emission scanning electron microscopy (FE‐SEM, Carl Zeiss, Germany).

    Article Title: An Intelligent Magneto-Mechanical Platform for Cellular Sensing in 3D Microenvironments.
    Article Snippet: .. In order to label the filamentous actin (F-actin), we incubated the cells with 1 μg/mL Phalloidin-FITC (CA1620, 12 of 15 Solarbio, Beijing, China) at room temperature for 60 min. We labeled cell nuclei with 4 ′ ,6-diamidino-2-phenylindole (DAPI) (S2110, Solarbio, Beijing, China) for 5 min. All samples were imaged using a Zeiss LSM 710 confocal microscope. .. The SEM and EDS images were obtained by field-emission scanning electron microscopy (FE-SEM, Carl Zeiss, Germany).

    Confocal Laser Scanning Microscopy:

    Article Title: Targeting JAK2 with a multifunctional nanoinhibitor for long-lasting anti-inflammatory effects and bone repair.
    Article Snippet: Following this period, the cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 for 10 min. Nonspecific binding sites were blocked with 5% goat serum for 1 h. Subsequently, cells were incubated overnight at 4 °C with anti-OCN (23418-1-AP, 1:400, Proteintech). .. The next day, cells were incubated with CoraLite594-conjugated secondary antibody (SA00013-4, 1:200, Proteintech) and Phalloidin-FITC (CA1620, 1:200, Solarbio) in the dark for 1 h. Images were captured using CLSM. ..

    Article Title: Targeting JAK2 with a multifunctional nanoinhibitor for long-lasting anti-inflammatory effects and bone repair
    Article Snippet: Following this period, the cells were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.1% Triton X-100 for 10 min. Nonspecific binding sites were blocked with 5% goat serum for 1 h. Subsequently, cells were incubated overnight at 4 °C with anti-OCN (23418-1-AP, 1:400, Proteintech). .. The next day, cells were incubated with CoraLite594-conjugated secondary antibody (SA00013-4, 1:200, Proteintech) and Phalloidin-FITC (CA1620, 1:200, Solarbio) in the dark for 1 h. Images were captured using CLSM. ..

    Staining:

    Article Title: Loss of <scp>SMN</scp> Impairs Osteoblast–Osteoclast Coupling via <scp>IGF1</scp>–Akt–<scp>OPG</scp> Axis in Spinal Muscular Atrophy
    Article Snippet: Multinucleated osteoclasts were identified using TRAP staining (Sigma- Aldrich, 387A) according to the manufacturer's instructions. .. For cytoskeleton visualization, cells were stained with phalloidin- FITC (Solarbio, China) and counterstained with DAPI (1:500, Beyotime, China) to visualize nuclei. .. Cells were imaged using a fluorescence microscope, and staining analysis was quantified with Image J software (RRID:SCR_003070), with results presented as bar graphs using GraphPad Prism 9.0 (RRID:SCR_002798).

    Article Title: Finerenone ameliorates high-fat-induced myocardial lipotoxicity by suppressing ferroptosis through augmenting the system Xc - /GSH synthesis pathway.
    Article Snippet: Obesity cardiomyopathy (OCM) is a distinct clinical entity in cardiovascular disease, yet targeted therapeutic options are scarce.. Finerenone, a novel nonsteroidal mineralocorticoid receptor antagonist, has shown cardioprotective effects, but its role in mitigating OCM lipotoxicity is unclear.. To elucidate the underlying functions and mechanisms, rats were fed on an HFD for 16 weeks to establish the OCM model. At the 12th week of HFD, the OCM rats were administered 10 mg/kg finerenone for 4 weeks.

    Article Title: Loss of SMN Impairs Osteoblast–Osteoclast Coupling via IGF1 –Akt– OPG Axis in Spinal Muscular Atrophy
    Article Snippet: Multinucleated osteoclasts were identified using TRAP staining (Sigma‐Aldrich, 387A) according to the manufacturer's instructions. .. For cytoskeleton visualization, cells were stained with phalloidin‐FITC (Solarbio, China) and counterstained with DAPI (1:500, Beyotime, China) to visualize nuclei. .. Cells were imaged using a fluorescence microscope, and staining analysis was quantified with Image J software (RRID:SCR_003070), with results presented as bar graphs using GraphPad Prism 9.0 (RRID:SCR_002798).

    Article Title: Optimized Design of a Magnetic-Controlled Transportation Tool for Directional Cell Migration.
    Article Snippet: To address the challenge of efficiently guiding directional cell migration, this study optimizes the design of a magnetically controlled transportation tool for cell migration.. The magnetic carriers are fabricated using digital light processing, with biocompatibility, magnetic particle dispersion, mechanical properties, magnetism, and printability serving as the selection criteria.. Based on these evaluations, a base material and a magnetic particle content of 30 wt.% are determined.



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    Servicebio Inc fitc conjugated phalloidin
    In vivo protective effects of RNM composite gel (A) Schematic of the in vivo experimental timeline and anatomical images of the mouse cochlea under a dissection microscope. (a, oval window; b, cochlear labyrinth.). (B–G) ABR tests performed 2 days before radiation exposure and 3, 7, and 14 days after drug administration to assess hearing function in mice. (H) Left: Confocal microscopy images of the cochlear basilar membrane 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel, stained with <t>FITC-phalloidin</t> to label hair cells. Scale bars, 20 μm. Right: Quantitative comparison of outer hair cell (OHC) and inner hair cell (IHC) survival rates post-intervention. Data are represented as the mean ± SEM ( N = 3, t test). (I) H&E staining images of mouse cochlear tissues extracted 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel. Data are represented as the mean ± SEM ( N = 3, t test). Scale bars, 20 μm. Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.
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    Image Search Results


    In vivo protective effects of RNM composite gel (A) Schematic of the in vivo experimental timeline and anatomical images of the mouse cochlea under a dissection microscope. (a, oval window; b, cochlear labyrinth.). (B–G) ABR tests performed 2 days before radiation exposure and 3, 7, and 14 days after drug administration to assess hearing function in mice. (H) Left: Confocal microscopy images of the cochlear basilar membrane 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel, stained with FITC-phalloidin to label hair cells. Scale bars, 20 μm. Right: Quantitative comparison of outer hair cell (OHC) and inner hair cell (IHC) survival rates post-intervention. Data are represented as the mean ± SEM ( N = 3, t test). (I) H&E staining images of mouse cochlear tissues extracted 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel. Data are represented as the mean ± SEM ( N = 3, t test). Scale bars, 20 μm. Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

    Journal: iScience

    Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

    doi: 10.1016/j.isci.2026.115723

    Figure Lengend Snippet: In vivo protective effects of RNM composite gel (A) Schematic of the in vivo experimental timeline and anatomical images of the mouse cochlea under a dissection microscope. (a, oval window; b, cochlear labyrinth.). (B–G) ABR tests performed 2 days before radiation exposure and 3, 7, and 14 days after drug administration to assess hearing function in mice. (H) Left: Confocal microscopy images of the cochlear basilar membrane 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel, stained with FITC-phalloidin to label hair cells. Scale bars, 20 μm. Right: Quantitative comparison of outer hair cell (OHC) and inner hair cell (IHC) survival rates post-intervention. Data are represented as the mean ± SEM ( N = 3, t test). (I) H&E staining images of mouse cochlear tissues extracted 14 days after radiation exposure and intratympanic injection of RN, MSCs, or RNM hydrogel. Data are represented as the mean ± SEM ( N = 3, t test). Scale bars, 20 μm. Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

    Article Snippet: The whole-mount basilar membranes were stained with FITC-conjugated phalloidin (Catalog #G1248-100T, Servicebio) and observed under a laser scanning confocal microscope for hair cell morphology assessment and counting.

    Techniques: In Vivo, Dissection, Microscopy, Confocal Microscopy, Membrane, Injection, Staining, Comparison